About

Splicify is a project designed to dramatically reduce the amount of time molecular biologists spend on designing gibson assembly reactions and improving the probability of successful cloning. It uses AI to decipher the user's intention and describe the logic used in the design and future steps for their experiment. All workflows are hard coded and deterministic with a workflow trace attached to the reply that allows our scientists to reliably replicate previous designs. The algorithm for designing primers utilizes primer3 to calculate primer characteristics and carefully considers the optimal extensions to maximize the probability of successful PCR and assembly. This algorithm incorporates annealing sequence Tm, overlap Tm, potential for mispriming or primer dimers, secondary structures that reduce the success of PCR, number of fragments, and length creating a full picture of the factors contributing to experimental success.

Open-Source Acknowledgements

This project builds on and is inspired by several outstanding open-source tools developed by the scientific software community. In particular, we acknowledge Primer3 (primer3-org) for foundational primer design algorithms; SeqViz (Lattice-Automation) for interactive DNA sequence visualization; repp (Lattice-Automation) for repository-based plasmid design and assembly logic; and pLannotate (Barrick Lab) for automated plasmid annotation workflows. We are grateful to the authors and contributors of these projects for making their work openly available and for advancing the molecular biology and synthetic biology ecosystem.

Contact

This application was created by Devon Fitzpatrick with advice on automation and business development from Rishij Mewada and with help from many friends in the molecular biology community!